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Oocytes expressing a probe for active Rho (rGBD) and various combinations of Ect2ΔNLS and RGA-3/4WT. Corresponds to Fig. 4, A–D. Time-lapse confocal microscopy; time in min:s since start of recording; 9, 7, 10, and 9 s/frame (Fig. 4, A–D) at 20 fps. Cells coexpressing Ect2ΔNLS and RGA-3/4WT show marked increase in excitable dynamics. Scale bar = 50 µm.
