TABLE 2.
The selected DEGs between 3D and 2D hMSCs from RNA‐seq
| Category | Symbols | Name | Log FC | P value |
|---|---|---|---|---|
| Cytoskeleton | TUBA1B | tubulin alpha 1b | −3.24 | 4.60E‐43 |
| TUBB2A | tubulin beta 2A class IIa | −2.76 | 2.77E‐29 | |
| ACTA2 | actin alpha 2, smooth muscle | −4.47 | 4.88E‐27 | |
| ACTC1 | actin alpha cardiac muscle 1 | −5.93 | 1.49E‐17 | |
| ACTB | actin beta | −2.41 | 3.00E‐31 | |
| ACTG1 | actin gamma 1 | −2.06 | 2.53E‐16 | |
| ECM | CDH1 | cadherin 1 | −1.33 | 3.77E‐02 |
| CDH2 | cadherin 2 | −1.36 | 4.98E‐04 | |
| CLDN1 | claudin 1 | −1.91 | 7.51E‐03 | |
| CLDN10 | claudin 10 | 3.15 | 2.36E‐04 | |
| MMP1 | matrix metallopeptidase 1 | 5.62 | 6.65E‐18 | |
| MMP13 | matrix metallopeptidase 13 | 6.72 | 2.77E‐33 | |
| MMP3 | matrix metallopeptidase 3 | 5.46 | 6.02E‐08 | |
| MMP9 | matrix metallopeptidase 9 | 5.92 | 5.91E‐08 | |
| Cytokine | FGF1 | fibroblast growth factor 1 | 1.21 | 1.36E‐03 |
| FGF13 | fibroblast growth factor 13 | 2.54 | 2.78E‐02 | |
| IL10 | interleukin 10 | 3.92 | 2.38E‐03 | |
| TGFB1 | transforming growth factor beta 1 | 1.64 | 1.18E‐05 | |
| TGFB2 | transforming growth factor beta 2 | 1.13 | 8.57E‐04 | |
| VEGFA | vascular endothelial growth factor A | 2.51 | 8.89E‐14 | |
| EV biogenesis | RAB27B | RAB27B, member RAS oncogene family | 3.37 | 2.08E‐20 |
| CD9 | CD9 molecule | 1.41 | 6.58E‐03 | |
| SDCBP | syndecan binding protein | 0.90 | 1.62E‐02 | |
| VPS37B | VPS37B subunit of ESCRT‐I | 0.75 | 6.88E‐03 | |
| CHMP4A | charged multivesicular body protein 4A | −0.44 | 4.28E‐02 | |
| CHMP7 | charged multivesicular body protein 7 | 0.68 | 6.51E‐05 | |
| CERK | ceramide kinase | 1.36 | 3.14E‐09 |
Note: The numbers are the Log2 values of 3D to 2D hMSCs. Negative values (Green) indicate that the genes are present in higher amounts in the 2D group, while positive values (Red) indicate that the genes are present in higher amounts in the 3D group. DEG: differentially expressed gene.