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. Author manuscript; available in PMC: 2022 Nov 19.
Published in final edited form as: ACS Chem Biol. 2021 Oct 22;16(11):2244–2254. doi: 10.1021/acschembio.1c00379

Figure 5. Cleavage on the C-terminal side of Gln in Ac-FAQDA, Ac-FAQPA and Ac-FAQAA.

Figure 5.

a) Time course of formation of Ac-FAQ from Ac-FAQDA and Ac-FAQPA following incubation at pH 4.0, 60°C. No cleavage of Ac-FAQAA was observed at pH 4.0. b) Apparent rate of formation of Ac-FAQ from Ac-FAQDA and Ac-FAQAA incubated at pH 6.7, 7.0 and 7.4. No cleavage of Ac-FAQPA was observed at these pHs. Cleavage rate was determined by the slope of the line over 25 days at 60°C. All samples were run in triplicate. Error bars +/− SD.