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. 2022 Jun 17;22:669. doi: 10.1186/s12885-022-09725-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — BM-like culture conditions increase CXCR4 surface expression and activate AKT and ERK pathways. A) Flow cytometry histograms of CXCR4 expression in LAN-1 cells cultured under BM-like conditions for 72 h (left). CXCR4 expression is represented as a fold-change to CM-CNT Nx (n = 3; right). B) Flow cytometry histograms of CXCR4 expression in SH-SY5Y cells cultured under BM-like conditions for 72 h (left). CXCR4 expression is represented as a fold-change to CM-CNT Nx (n = 3; right). Histograms were generated with NovoFlow Software (Acea Bioscience). Normoxia results are in black and hypoxia in blue. (*) P < 0.01 (Two-way ANOVA, Dunnett’s multiple comparison test). Immunoblots of LAN-1 C) and SH-SY5Y D) cultured under BM-like conditions for 48 h. Band intensity p-AKT (S473), p-ERK (T202/Y204 T185/Y187) ratios are calculated by measuring the relative protein levels (phosphorylated/total), and γ-Tubulin (as a loading control) by using ImageJ Software (n = 2)