Table 1.

Sequences of primers for detection of HBV genotypes and mutations

Virus	Primers name	Sequences of Primers 5′ → 3′	Gene	Region in genome	Annealing temperature	Size	References
HBV	244-HBS-F1	GAGTCTAGACTCGTGGTGGACTTC	S	244–267	56 ℃	447 bp	[19–21]
	691-HBS-R1	AAATKGCACTAGTAAACTGAGCCA		668–691
	255-HBS-F2	CGTGGTGGACTTCTCTCAATTTTC		255–278	56 ℃	416 bp
	671-HBS-R2	GCCARGAGAAACGGRCTGAGGCCC		648–671
HBV	1606-pre-C-F1	GCATGGAGACCACCGTGAAC	X and pre-core regions	1606–1625	57 ℃	789 bp	[21–23]
	2395-pre-C-R1	AGGCGAGGGAGTTCTTCTTC		2376–2395
	1653-pre-C-F2	CATAAGAGGACTCTTGGACT		1653–1672	55 ℃	740 bp
	2393-pre-C-R2	GCGAGGGAGTTCTTCTTC		2376–2393