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. 2022 Jun 18;14:84. doi: 10.1186/s13195-022-01022-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — NIR light activation phagocytosis of Aβ-treated microglia cells. Changes in the fluorescence signal from Aβ following stimulation by non-fluorescent Aβ, low-intensity light at 808 nm with 10 J/cm², and both Aβ and light over time was recorded. The representative transmission microscopy images show phagocytosis of Aβ by microglia after 30 min, 2 h, 4 h, 16 h, and 24 h. The enlarged brightfield and merged images are presented in Fig. S4, Supplementary Materials. The data are presented as the mean ± SD (n = 6 replicates in each group); *p < 0.05 indicates data with a statistically significant difference evaluated in relation to the control level, and ^#p < 0.05 indicates data with a statistically significant difference evaluated in relation to the level during co-cultivation with Aβ-treated microglia (two-way ANOVA test)