Fig. 7.

Increased neuron cell viability after NIR exposure in a co-culture with Aβ stimulated microglia. A Schematic illustration of neuron cell cultivation in the Boyden chamber under different conditions, from left to right: control, light application (808 nm, 10 J/cm²), Aβ addition, co-cultivation with microglia (M), co-cultivation with Aβ-treated microglia (Aβ-M), co-cultivation with Aβ-M after light application. B Epifluorescence microscopy images showing the effect of 808 nm light, Aβ, co-cultivation with M, co-cultivation with Aβ-M, co-cultivation with Aβ-M + 808 nm light on neuron cell viability with Calcein (green) to visualize the live cells, Annexin (blue) for apoptosis, and Propidium Iodide (red) for necrosis detection during 48 h. The enlarged images are presented in Fig. S7, Supplementary Materials. C Columns present the quantification of viable, apoptotic and necrotic neuron cells. The data are presented as the mean ± SD (n = 8 replicates in each group); *p < 0.05 indicates data with a statistically significant difference evaluated in relation to the control level, and ^#p < 0.05 indicates data with a statistically significant difference evaluated in relation to the level during a co-cultivation with Aβ-treated microglia (two-way ANOVA test)