TABLE 1.
Specificity of nested set RT-PCR for detection of C. parvum oocysts
| Organism (source) | Results of lateral flow detectiona |
|---|---|
| Cryptosporidium parvum KSU-1 (S. Upton, KSU)b | Positive |
| Cryptosporidium parvum KSU-4 (S. Upton, KSU) | Positive |
| Cryptosporidium muris (S. Upton, KSU) | Negative |
| Cryptosporidium serpentis (S. Upton, KSU) | Negative |
| Cryptosporidium sp. strain KSU-3 (S. Upton, KSU) | Negative |
| Cryptosporidium sp. (S. Upton, KSU) | Negative |
| Escherichia coli DH5α (U. Ochsner, UCHSC)c | Negative |
| Pseudomonas aeruginosa PA01 (U. Ochsner, UCHSC)c | Negative |
a
Lateral flow detection was performed after lysis and extraction of oocysts followed by amplification as described in Materials and Methods.
b
KSU, Kansas State University, Manhattan; UCHSC, University of Colorado Health Sciences Center, Denver.
c
Purified chromosomal DNA of this organism was tested directly by using the nested set RT-PCR.