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. Author manuscript; available in PMC: 2022 Jun 18.
Published in final edited form as: Toxicol Lett. 2021 Jan 6;340:33–42. doi: 10.1016/j.toxlet.2021.01.005

Fig. 6.

Fig. 6.

Cytoprotective effects of N-acetylcysteine (NAC) against acrolein exposure in hCSFs. Dose-dependent response of NAC using MTT assay was performed and dose upto 1 mM was found to be safe in hCSFs (A). The phase-contrast live bio-microscopic images showed that NAC at 1 mM concentration reduced (E) the adverse effects of acrolein exposure (ACR) on cellular viability in hCSFs (D). On the other hand, NAC treatment alone (C) showed similar cellular morphology to control group (B). All the experiments were independently performed three times using each sample in triplicates. The results were presented as mean ± SEM. The one-way analysis of variance (ANOVA) with Bonferroni post hoc test were used for statistical analysis. Scale bar = 100 μm.