Skip to main content
. Author manuscript; available in PMC: 2022 Jun 18.
Published in final edited form as: Exp Eye Res. 2019 Jan 3;180:200–207. doi: 10.1016/j.exer.2019.01.001

Fig. 6. Decorin-mediated EGFR internalization does not involve early endosome.

Fig. 6.

Human CSFs were incubated with rhDcn (250 nM, A-D) or rhEGF (100 ng/ml, E-H). Double immunostaining of hCSFs with EGFR (green, A and E) and early endosome marker, EEA1, (red, B and F) showed EGFR and EEA1 co-localization vesicle only with rhEGF treatment (G, arrows). On the other hand, rhDcn-treatment to hCSF cells showed insignificant co-localization of EGFR and EEA1 (C). Schematic diagram shows internalization does not target early endosome (EEA1) upon decorin treatment (D) but it targets EEA1 when treated with EGF (H). Bar = 10 μM. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)