Figure 4.

In vivo fluorescein dye images showing a highly diffuse uptake of dye in the affected areas in the rabbit eyes after acrolein vapor exposure (1 min, B–F; and 3 min, H–L) and mildly diffused after acrolein topical exposure (1 min, N–R; and 5 min, T–X) at the tested times (30 min to 28 days). Vapor exposure to the cornea caused severe corneal abrasions and corneal surface defects, which fail to heal even at day 28 (F and L). A similar pattern was observed after topical exposure, with areas of severe corneal abrasions (N–P and T–V) that heal at a delayed rate up to day 28 (Q and R, and W and X). n = 6 rabbits in each group. All images were taken at 7.1× magnification. Scale bar = 3.0 mm.