A) Western blot analysis shows expression of LC3A/B, p62 and LAMP1 in normal (control) and wounded (scratch) HSF cells. B) Densitometric analysis of LC3B, p62 and LAMP1 proteins. GAPDH served as the loading control and was used for densitometric analyses. C) Ratio of LC3B and p62. Data are expressed as mean ± SD, n = 3, statistical significance was denoted by * (p < 0.05) and ns (not significant as compared to control).
(GAPDH: glyceraldehyde 3-phosphate dehydrogenase; HSF: human stromal fibroblast. LAMP1: lysosomal-associated membrane protein 1; LC3: microtubule associated protein 1 light chain 3 alpha).