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. Author manuscript; available in PMC: 2022 Jun 18.
Published in final edited form as: Ocul Surf. 2019 Jan 25;17(2):186–197. doi: 10.1016/j.jtos.2019.01.008

Table 2.

Primers used for PCR analysis.

Autophagy-related gene Gene sequence (5’–3’)

LC3 F: TCCTGTACATGGTCTATGCC
R: CGTTTACCCTGCGTTTGT
BECN1 F: CTGGACAAGCTGAAGAAGAC
R: TGTGAGGATACCCAAGCA
Atg5 F: GAAGGCACACCACTGAAA
R: AGCTCAGATGCTCACTCA
DRAM1 F: GTCAGCCGCCTTCATTAT
R: TCCACTCCAGCTTGGTTA
SQSTM1 F: GGTGAAACACGGACACTT
R: GACTGGAGTTCACCTGTAGA
PRKN2 F: GCACCTGATCGCAACAAA
R: CTCTGTAGGCCTGAGTAGTT

(Atg5: autophagy-related protein 5; BECN1: Beclin 1; DRAM1: DNA damage regulated autophagy modulator 1; LC3: microtubule associated protein 1 light chain 3 alpha; PRKN2: E3 ubiquitin-protein ligase parkin; SQSTM1: sequestosome 1).