FIG. 2.

Detection of Lon from Pf-5 grown in M9 minimal medium (45) with antiserum to Lon of E. coli. Sample numbers correspond to extracts from cells growing exponentially (EX) at 27°C at an OD₆₀₀ of 0.2 (lanes 1, 2, and 6), cells heat shocked (HS) at 42°C for 25 min (lanes 3, 7, and 10), early-stationary-phase cultures (ES) at an OD₆₀₀ of 2 (lanes 4 and 8), and cultures grown overnight to a final OD₆₀₀ of 2 to 4 (late stationary phase [LS]) (lanes 5 and 9). Lanes: 1, E. coli SG20781; 2 to 5, Pf-5; 6 to 9, JL3985 (rpoS::Tn5); 10, JL4292 (lon::Tn5). Experiments were repeated with similar results. Scanned images were prepared for publication using Adobe Photo Shop version 4.0 (Adobe Systems Incorporated, San Jose, Calif.).