Skip to main content
. 2022 Jun 18;8:293. doi: 10.1038/s41420-022-01082-6

Fig. 2. CC-115 provokes apoptosis in primary human NSCLC cells.

Fig. 2

pCan1 cells were treated with CC-115 (100 nM) and cultivated for the designated time periods, the relative caspase-3 and caspase-7 activities A, B and expression of apoptosis-related proteins C were examined; Cell apoptosis was measured using the nuclear TUNEL staining D and Annexin V flow cytometry E assays. pCan1 cells were pretreated for 45 min with the designated caspase inhibitors (each at 50 μM) or 0.25 % DMSO, followed by CC-115 (100 nM, 96 h) treatment, cell viability F and cell death G were examined. For each assay, n = 5. *P < 0.05 versus “Veh” group. #P < 0.05 versus “DMSO” pretreatment F, G. Scale bar = 100 μm D.