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. Author manuscript; available in PMC: 2022 Jun 19.
Published in final edited form as: Zoonoses (Burlingt). 2022 Jun 14;2(1):18. doi: 10.15212/zoonoses-2022-0012

Figure 4. BRBV plaque assay.

Figure 4.

A BRBV stock was diluted from 10 −4 to 10−9 in Dulbecco’s Modified Eagle’s medium (DMEM) media. 1 ml of each diluted virus were added in one well of a 6-well plate of Vero cells. After incubation of 1 hour at 37°C, diluted virus-containing media were removed from each well. 2 ml of DMEM with 10% fetal calf serum and 1% methylcellulose overlay were added to each well. After 3 days post-infection, the media was aspirated from each well, and the cells were stained with crystal violet [30]. Plaques, foci of infected cells, are shown as white dots in the stained cell sheet (blue background).