Fig. 7.

Effect of secretome from from IL-4- or IFN-γ-treated microglia on survival and proliferation via PI3K/Akt pathway in adult NSPCs. A Fluorescence micrographs of apoptotic cells from NSPCs differentiate in PBS, IL-4 or IFN-γ or microglia-conditioned medium for 7 days. The apoptotic cells were stained with Cleaved-caspase 3 (green) by immunocytochemistry and nucleus is labeled by DAPI (blue). Scale bar is 10 μm. B The histogram represents quantification of percentage of CC-3⁺ cells. Data are showed Mean ± SEM, n = 4–6, *P < 0.05 vs PBS group, ^###P < 0.001 vs IL-4 group