Figure 1.

Gating strategy for analysis using logic gates. Measurements were performed with fresh blood samples. (a) Tfh cells were defined as CD4⁺CXCR5⁺ T cells in the lymphocyte population. The Tfh cell phenotypes were PD-1⁺ICOS⁺CD4⁺CXCR5⁺ T cell (PD-1⁺ICOS⁺Tfh cells), CD226⁺CD4⁺CXCR5⁺ T cell (CD226⁺Tfh cells), and TIGIT⁺CD4⁺CXCR5⁺ T cell (TIGIT⁺Tfh cells). (b) B cells were further defined as CD19-expressing cells in the lymphocyte population. CD19⁺ B cells were analyzed for the expressions of IgD and CD27, CD24, and CD38. The B cell populations included CD27⁻IgD⁺ (naïve B cells), CD27⁺IgD⁺ (unswitched memory B cells), CD27⁺IgD⁻ (switched memory B cells), CD24⁺⁺CD38⁺⁺ (transitional B cells), CD27⁺CD38⁺ (plasmablasts), and CD38⁺ B cells (plasma cells).