Fig. 5.

Pretreatment with reactive oxygen scavengers inhibits the IL-13-induced cellular senescence. a, b SMG-C6 cells were pretreated with NAC (10 μmol·L⁻¹) (a) and MitoTEMPO (1 μmol·L⁻¹) (b) for 30 min, and then incubated with IL-13 (50 ng·mL⁻¹) for 4 days. The cellular senescence was measured by SA-β-gal staining (n = 6 per group). c, d SMG-C6 cells were pretreated with MitoTEMPO (1 μmol·L⁻¹) for 30 min, and then incubated with IL-13 (50 ng·mL⁻¹) for 4 days. The mRNA (c) and protein (d) expression of p53, p21, and p16 were detected by using quantitative real-time PCR and western blot, respectively (n = 6 per group). The significance of differences between groups was analyzed by one-way ANOVA followed by Bonferroni’s test. Data are presented as the mean ± SD