TABLE 2.

Bacterial growth and β-galactosidase activity of P. putida 06909u2 containing different plasmids

Introduced plasmid or prepn	Bacterial growth on V8C agar		β-Galactosidase activity (Miller units)
	Without fungusa	With fungusb	V8CU brothc	V8CU agard	V8C agar fungal disc
pRIV13	+	+	NDe	ND	ND
pRIV16	−	−	49	26	ND
pRIV16C	+	+	1,570	4,375	2,166
pRIVA1	+	+	3,009	933	3,005
pRIVS1	−	+	237	189	3,297
pRIVS2	−	+	201	142	2,597
pRIVS3	−	+		371	3,957
pRIVS4	−	+	166	144	731
pRIVS5	−	+	306	141	1,834
Fungal disc			ND	ND	ND

^aBacterial growth on a V8C agar plate. −, no visible bacterial colonies after 2 days; +, visible bacterial growth after 1 day. 

^bBacterial growth on a fungal disc as determined by turbidity. −, A₆₀₀ less than 0.075; +, A₆₀₀ more than 0.15. 

^cV8CU broth, V8C broth supplemented with uracil (50 μg/ml). 

^dV8CU agar, V8C agar supplemented with uracil. 

^eND, not detected.