Figure 5.

Knockdown of CaMK4 inhibited NLRP3 inflammasome activation in type II lung alveolar epithelial cells. CaMK4 expression in A549 cells were knocked down using CaMK4 specific siRNA and stimulated with LPS+ATP or PBS. (A–D) CaMK4, NLRP3, Caspase-1p20 and GAPDH expression was measured by Western blot. Representative bands were shown and relative expression summarized from 3 independent experiments. (E) IL-1β in supernatants from A549 cells was determined by ELISA. All data are mean± SEM. ns, not significant. **p < 0.01, ***p < 0.001.