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PHP.S-tdTf transduced nerve fibers in the margins of mouse proximal colon combined with immunofluorescent labeling. PHP.S-tdTf (1 x 1012 GC/mouse) was retro-orbitally injected 3 weeks before. Upper row: the mesenteric margin enriched by nerve fibers, and many dual-labeled by PHP.S-tdTf and TH. Middle row and lower row: the antimesenteric margin consisted of small circumferential nerve bundles and scattered neurons immunostained by PGP9.5 or nNOS (arrows). Scales 100 μm for each row.
