Figure 3.

AAV9-transduced neurons in the myenteric plexus in whole mount preparations of the mouse proximal colon. AAV9-CAG-GFP was retro-orbitally injected at 3.3 x 10¹¹ GC/mouse 3 weeks before. (A) Axons of a few large neurons project out of the ganglia in different directions, and many neurons are small and medium in size without labeled processes. (B) One neuron has a large soma and a thick varicose axon (arrow). (C) The axon of a big neuron has enlarged initial part (arrow) and varicosities en route. (D) The arrow points to some short thick dendrites of one neuron at the edge of one ganglion. Scales as indicated in each image.