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. 2022 Jun 6;16:884280. doi: 10.3389/fnana.2022.884280

Figure 4.

Figure 4

Dual labeling of AAV9 transduction and immunofluorescence of pan-neuronal markers, NeuN in the submucosal plexus (left panels) and HuC/D myenteric plexus (right panels) in whole mount preparations of the mouse proximal colon. AAV9-CAG-GFP was retro-orbitally injected at 3.3 x 1011 GC/mouse 3 weeks before. The submucosal neurons were labeled by NeuN, due to a lot of non-specific labeling by the HuC/D antibody.