Figure 4.
Dual labeling of AAV9 transduction and immunofluorescence of pan-neuronal markers, NeuN in the submucosal plexus (left panels) and HuC/D myenteric plexus (right panels) in whole mount preparations of the mouse proximal colon. AAV9-CAG-GFP was retro-orbitally injected at 3.3 x 1011 GC/mouse 3 weeks before. The submucosal neurons were labeled by NeuN, due to a lot of non-specific labeling by the HuC/D antibody.