Figure 6.

AAV9 transduction in the myenteric plexus whole mount preparations of the mouse proximal colon combined with immunofluorescence of glial fibric acid protein (GFAP) for glial cells (left panels) and ionized calcium binding adaptor molecule 1 (Iba1) for macrophages (right panels). AAV9-CAG-GFP was retro-orbitally injected at 3.3 x 10¹¹ GC/mouse 3 weeks before. No AAV9-transduced cells were GFAP-ir or Iba1-ir. White arrow indicate GFAP-ir glial cell bodies, and blue ones indicate AAV9 transduced non-neuronal cells which were not GFAP-ir. Many Iba1-ir cells were located near or inside the ganglia and interganglionic strands (bottom-right panel).