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. 2022 Jun 6;16:884280. doi: 10.3389/fnana.2022.884280

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Copyright © 2022 Wang, Yuan, Challis, Ravindra Kumar and Taché.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AAV9 transduction in the myenteric plexus whole mount preparations of the mouse proximal colon combined with immunofluorescence of tyrosine hydroxylase (TH, left panels) and calcitonin gene related peptide-alpha (αCGRP, right panels). AAV9-CAG-GFP was retro-orbitally injected at 3.3 x 10¹¹ GC/mouse 3 weeks before. TH and αCGRP immunofluorescence (red) barely co-existed with AAV9 (green). Notably, αCGRP immunofluorescent nerve fibers surrounded neuronal somas more frequently (arrows) than TH.