Figure 8.

c-Kit-immunofluorescent cells (red) in the mouse proximal colon with AAV9 transduction (green). AAV9-CAG-GFP was retro-orbitally injected at 3.3 x 10¹¹ GC/mouse 3 weeks before. (A) c-Kit-ir cells located near the myenteric plexus transduced by AAV9; (B) In the longitudinal muscle layer, not all of the c-Kit-ir elongated cells with processes were aligned parallelly to the projection of AAV9-transduced nerves (arrows); a few varicosities closely located to c-Kit-ir cells (yellow dots marked by arrowheads); (C) AAV9-transduced nerve fibers were distributed among c-Kit-ir cells in the base of the mucosal crypts; (D) c-Kit cells closely located to the submucosal plexus. Scales as indicated in each image.