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. 2022 Jun 6;9:828240. doi: 10.3389/fmed.2022.828240

Figure 3.

Figure 3

Effect of HG on expression of circ_0004951 and pyroptosis of HK2 cells. qRT-PCR results showing that HG mediated a marked upregulation of circ_0004951 and NLRP3 with a downregulation of miR-93-5p (A–C). CCK-8 assay results showing that HG suppressed HK2 cell viability compared to NG or HO groups (D). HG increased LDH release (E). HG increased the caspase-1 activity (F). ELISA results showing that HG upregulated IL-1β (G) and IL-18 (H) expression. Western blots showing that HG upregulated IL-1β, IL-18 and NLRP3 expression (I). HG, high glucose; NG, normal glucose (control); HO, hyperosmotic; HK2, human renal tubular epithelial cell line; qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, cell counting kit-8; LDH, lactate dehydrogenase; NLRP3, NLR pyrin domain containing 3. Values are expressed as mean ± SD. *p < 0.05 vs. control.