Skip to main content
. 2022 Jun 6;9:828240. doi: 10.3389/fmed.2022.828240

Figure 4.

Figure 4

The regulatory relationship between circ_0004951, miR-93-5p and NLRP3. The expression of circ_0004951, parental gene TRPM7, miR-93-5p and NLRP3 after knockdown of circ_0004951 (A). MiR-93-5p mimic and miR-93-5p inhibitor upregulated and downregulated expression of miR-93-5p and NLRP3, respectively (B). Bioinformatics analysis showed the adsorption and binding sites for miR-93-5p in circ_0004951 (C), and seed region sites of NLRP3 3′UTR bound to miR-93-5p (D). Luciferase reporter assay results showing relative luciferase activities after co-transfection of construct containing wt or mut of circ_0004951 and miR-93-5p mimics or inhibitors (E). Luciferase reporter assay results showing luciferase activities after co-transfection with the miR-93-5p mimic containing either wt or mut of NLRP3 (F). NLRP3, NLR pyrin domain containing 3; HK2, human renal tubular epithelial cell line; 3′-UTR, 3′-untranslated region. Values are expressed as mean ± SD. *p < 0.05 vs. control.