Table 1.
Highly upregulated sporozoite genes candidate list.
| Gene ID | Product Description | Other Information, Homologies, and Domains | OO4 | OO10 | OO0 | MZ* | TZ | BZ |
|---|---|---|---|---|---|---|---|---|
| TGME49_203890 | hypothetical protein | 358 | 201 | 31 | 4 | 0 | 0 | |
| TGME49_206400 | FAD binding domain of DNA photolyase domain-containing protein | 1361 | 642 | 101 | 17 | 1 | 1 | |
| TGME49_209920 | PAN domain-containing protein | MIC4L | 965 | 237 | 0 | 1 | 0 | 4 |
| TGME49_214570 | hypothetical protein | Nuclear pore glycoprotein domain | 370 | 300 | 20 | 0 | 0 | 1 |
| TGME49_220280 | mucin family glycoprotein | 240 | 157 | 0 | 1 | 4 | 3 | |
| TGME49_223430 | hypothetical protein | 148 | 23 | 0 | 2 | 1 | 14 | |
| TGME49_226230 | hypothetical protein | 55 | 42 | 0 | 0 | 0 | 0 | |
| TGME49_227100 | hypothetical protein | Putative Glutaredoxin | 12391 | 7405 | 82 | 30 | 1 | 76 |
| TGME49_258200 | hypothetical protein | Putative glutamic acid rich protein | 206 | 166 | 0 | 1 | 1 | 3 |
| TGME49_258550 | SAG-related sequence SRS28 | SporoSAG | 14087 | 3744 | 3 | 3 | 5 | 4 |
| TGME49_258810 | SAG-related sequence SRS27B | 373 | 117 | 0 | 4 | 0 | 26 | |
| TGME49_259670 | von Willebrand factor type A domain-containing protein | CTRP (CS protein-TRAP-related protein) | 4595 | 1270 | 0 | 4 | 0 | 0 |
| TGME49_265120 | rhoptry neck protein, putative | SporoRON2 | 70 | 50 | 0 | 1 | 1 | 2 |
| TGME49_269380 | hypothetical protein | Elongation factor 4 and GTP-binding protein domains | 84 | 74 | 0 | 0 | 1 | 0 |
| TGME49_270950 | hypothetical protein | Thymidylate kinase domain | 761 | 709 | 6 | 2 | 2 | 1 |
| TGME49_271210 | 4-alpha-glucanotransferase | 782 | 139 | 0 | 20 | 0 | 1 | |
| TGME49_272240 | Toxoplasma gondii family D protein | 584 | 458 | 1 | 0 | 0 | 0 | |
| TGME49_273510 | hypothetical protein | 369 | 243 | 0 | 0 | 0 | 2 | |
| TGME49_276850 | hypothetical protein | LEA-TgERP (Embryogenesis related protein) | 11753 | 3640 | 1 | 1 | 0 | 1 |
| TGME49_276860 | hypothetical protein | LEA (Late Embryogenesis Abundant protein) | 282 | 203 | 1 | 1 | 0 | 1 |
| TGME49_276870 | hypothetical protein | LEA (Late Embryogenesis Abundant protein) | 2354 | 499 | 23 | 1 | 1 | 1 |
| TGME49_276880 | hypothetical protein | LEA (Late Embryogenesis Abundant protein) | 4446 | 1240 | 5 | 0 | 0 | 1 |
| TGME49_278120 | SCP family extracellular subfamily protein | 531 | 326 | 0 | 3 | 1 | 1 | |
| TGME49_281590 | hypothetical protein | Putative periplasmic substrate binding protein | 119626 | 15174 | 5 | 2 | 2 | 13 |
| TGME49_292350 | hypothetical protein | Putative Toxoplasma Family A-likely surface antigen | 863 | 239 | 1 | 0 | 0 | 2 |
| TGME49_293620 | hypothetical protein | 48 | 26 | 0 | 0 | 0 | 2 | |
| TGME49_294600 | Toxoplasma gondii family D protein | Alpha-2U-globulin conserved domain | 3772 | 3004 | 7 | 5 | 0 | 1 |
| TGME49_297280 | hypothetical protein | 828 | 142 | 0 | 3 | 0 | 19 | |
| TGME49_309540 | hypothetical protein | 69 | 57 | 1 | 0 | 0 | 1 | |
| TGME49_315260 | alanine dehydrogenase | 185 | 330 | 33 | 36 | 11 | 14 | |
| TGME49_315730 | apical membrane antigen 1 protein | SporoAMA1 | 1247 | 357 | 68 | 27 | 0 | 35 |
| TGME49_319890 | hypothetical protein | Putative Tyrosine-rich protein | 38312 | 6594 | 0 | 2 | 10 | 3 |
| TGME49_320280 | hypothetical protein | Hemin storage protein, chitinase and glycosyl hydrolase domains | 85 | 68 | 0 | 0 | 0 | 1 |
| TGME49_320530 | hypothetical protein | Putative Tyrosine-rich protein | 6656 | 4086 | 2 | 0 | 0 | 0 |
Indicated are the gene IDs for which at least 10 unique peptides were detected in proteomic analysis of oocysts (59, 60) and 0 peptides in any other stages, and that had at least 4-fold higher expression in sporulated (day 4 and day 10 –OO4 and OO10-) vs. unsporulated oocysts (day 0 -OO0-) (70) and vs. tachyzoites (TZ), bradyzoites (BZ), and merozoites (MZ) (43). Numbers represent the average expression level in transcripts per million (TPM). *For the merozoite value, the average of the 5 Enteroepithelial stages (EES) described by Ramakrishnan et al. was used (43)