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. 2022 Jun 6;13:874056. doi: 10.3389/fimmu.2022.874056

Figure 12.

Figure 12

CX3CL1 Knockdown promoted migration and invasion of ccRCC cell in vitro. (A) Real-time RT-PCR assay of 10 LR-pairs mRNA expression levels in human ccRCC cell line 786-O and renal epithelial cell HK-2. (B, C) the protein expression of CX3CL1 was performed by Western Blot assay. (D) The subcellular localization of CX3CL1 was identified by immunostaining assay. Scale bar: 10μm. (E) Cell colonies of 786-O cell in normal control and CX3CL1-KD group. (F, G) 786-O cell migration assay (F) and invasion assay (G) were performed in normal control and CX3CL1-KD group. ns, p≥0.05; *p< 0.05; **p<0.01; ***p<0.001.