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Validation of the circular structure of circRNA. circRNAs exist in human myocardial cell line (AC16). Clear single bands were amplified from the cDNA of AC16 by divergent primers, while they could not be amplified from gDNA. Sanger-Seq validated the head-to-tail junction of circRNAs. circTMEM165 (A), circUBAC2 (B), circZNF609 (C), circANKRD12 (D), circSLC8A1 (E) exists in human myocardial cell.
