Figure 4.

Identifying signaling pathways in a myogenic cluster acquiring quiescence

(A) A Dotplot representing NicheNet-imputed ligands produced by mononuclear cells in regenerating skeletal muscle predicted to influence myogenic cells. The dot size reflects the precent gene expression, and the hue intensity depicts the relative gene expression.

(B) The most significantly expressed genes for cell ligands (by Pearson coefficient) and their potential interactions with genes encoding receptors on SCs determined by NicheNet. Hue indicates interaction potential, a representation of the number and quality of data sources supporting the ligand-receptor interaction.

(C) Expression levels for the most significant genes encoding ligands in mononuclear cells from regenerating skeletal muscle; the heatmap indicates the relative gene expression level. See also Figure S5, Table S7, and S8.