Figure 5.
The regenerating muscle environment controls SC cell fate
For a Figure360 author presentation of this figure, see https://doi.org/10.1016/j.isci.2022.104444.
(A) Hierarchical clustering of NicheNet-identified genes encoding receptors on myogenic cell clusters. The heatmap indicates the Z score.
(B) Schematic of experimental design for SC transplantation concomitant with injury, or at 5 dpi (during the peak of SC quiescence acquisition).
(C) Representative images of engrafted tdTomato+/Pax7+ SCs (filled arrowheads) at 14 days posttransplant. Scale bar: 100 μm.
(D) Quantification of tomato+, donor-derived, nuclei identified per mm2 from transplants at 0 dpi, 5 dpi, and into uninjured TA muscle. Statistical significance tested by ANOVA. ∗p value < 0.05.
(E) The percentage of donor cells engrafting either as myonuclei or as SCs. Statistical significance tested by t test with p value = 0.006, For all mean ± SD, n = individual mice, where all tomato + cells in each of three TA muscle sections were quantified.
(F) A model depicting the influence of the regenerating muscle microenvironment on SC cell fate. See also Figures S6 and S7.