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. 2022 Apr 15;113(6):2056–2070. doi: 10.1111/cas.15356

FIGURE 3.

FIGURE 3

Silence of cell migration‐inducing protein (CEMIP) attenuates antioxidant capacity of detachment‐resistant (DR) prostate cancer (PCa) cells by downregulating SLC7A11. (A) Western blotting analysis of SLC7A11 expression in the indicated parental and DR PCa cells. (B) Quantitative real‐time polymerase chain reaction (qRT‐PCR) analysis of the transcription levels of SLC7A11 in indicated parental and DR PCa cells. (C) Western blotting to analyze the change of SLC7A11 caused by CEMIP knockdown in the indicated DR PCa cells. (D) qRT‐PCR to analyze changes in the transcription level of SLC7A11 caused by CEMIP knockdown in the indicated DR PCa cells. (E, F) Expression levels of SLC7A11 comparison between prostate tumor samples and normal tissues from the TCGA; (E) unpaired; (F) paired. (G) Expression levels of SLC7A11 in prostate cancer patients with different Gleason scores from the TCGA. (H) Univariate Cox regression analysis of the progress‐free interval (PFI) in prostate cancer patients with different expression levels of SLC7A11 from the TCGA. (I) Western blotting analysis of SLC7A11 expression in PCa tissues (N, normal tissues; T, tumor tissues). (J) Left panel, immunohistochemistry (IHC) analysis of SLC7A11 expression difference between PCa tissues and corresponding pericarcinous specimens. Right panel, H‐scores of SLC7A11 among PCa tissues with different Gleason scores. (K) Intracellular cystine were detected by using a cystine‐FITC fluorescent probe in the presence of shCEMIP plasmids or oeSLC7A11 plasmids or both shCEMIP plasmids and oeSLC7A11 plasmids in the indicated DR PCa cells; confocal microscopy was used to record the fluorescence signal. Scale bars, 20 μm. (L) GSH/GSSH ratio was measured in the presence of shCEMIP plasmids or oeSLC7A11 plasmids or both shCEMIP plasmids, and oeSLC7A11 plasmids in the indicated DR PCa cells. (M) Cell viability was detected by CCK‐8 assay in the indicated group. Data are presented as representative images or as mean ± SD from three independent repeats. *p < 0.05, **p < 0.01, ***p < 0.001