FIGURE 2.
Low‐density lipoprotein (LDL) induces cholesterol accumulation in lymphoma cells. (A) Giemsa staining of human adult T‐cell leukemia/lymphoma cell line (ED), human B cell lymphoma cell line (DAUDI), and human primary central nervous system lymphoma cell lines (HKBML and TK). (B) Electron microscopy images of lymphoma cell lines. (C) Detection of lipid accumulation in lymphoma cell lines by Sudan black B staining. (D) Microscopy of Sudan black B stained cells to determine the lipid accumulation of lymphoma cells incubated with LDL (40 μg/ml) or high‐density lipoprotein (HDL; 40 μg/ml) for 24 h under lipoprotein‐deficient serum (LPDS, 2.5%) conditions. (E) Electron microscopy of DAUDI and TK cells treated with LDL (40 μg/ml) for 24 h. (F) Total cholesterol (chol) and free cholesterol of lymphoma cells following incubation with LDL (40 μg/ml) for 24 h. (G) 3[H] cholesteryl ester (CE) in lymphoma cells following incubation with LDL (40 μg/ml) in the presence of 3[H]oleate for 24 h