FIGURE 3.

Cytotoxic effect of neutrophils on tumor cells mediated by riluzole and glutamate. (A) Cell viability of LLC after coculturing with glutamate treated neutrophils at different ratios. (B) Cell viability of LLC after coculturing with riluzole pretreated neutrophils at different ratios. */**Significant differences between glutamate (100 μM) and blank neutrophil groups. (C) Cell viability of three tumor cell types after riluzole treatment for 24 h. (D–F) Cell viability of LLC/B16/MC38 cocultured with/without neutrophils at 1:10 ratio combined with gradient doses of CB‐839 treatments (0–2.5 μM) for 24 h. **Significant differences between tumor cell alone groups and neutrophil coculture groups. Riluzole (2.5 μM) or glutamate (100 μM) pretreated neutrophil/tumor cells for 6 h and resuspended with complete DMEM. Subsequently, these pretreated neutrophils/tumor cells were cocultured at different ratios for 24 h. Tumor cell viability was determined by luciferase signal intensity. (G–I) Cell viability of LLC/B16/MC38 after coculturing with neutrophils. */**Differences between riluzole group and blank group. #/##Differences between glutamate group and blank group. n = 5