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. 2022 Jun 6;13:858263. doi: 10.3389/fmicb.2022.858263

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Copyright © 2022 Kusada, Arita, Tohno and Tamaki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BSH activity and biochemical characterization of LpBSH. (A) BSH activity was measured against eight human bile salts: taurocholic acid (TCA), taurochenodeoxycholic acid (TCDCA), taurodeoxycholic acid (TDCA), tauroursodeoxycholic acid (TUDCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), glycodeoxycholic acid (GDCA), and glycoursodeoxycholic acid (GUDCA). Values are indicated as means for three independent experiments (n = 24, ^∗p < 0.05). Error bars represent standard deviation (SD). Effects of temperature (B) and pH (C) on BSH activity against TDCA. Each value is expressed as mean values for eight technical replicates (each n = 8). Maximum activity was taken as 100%. Error bars indicate SD.