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. 2022 Jun 6;13:858263. doi: 10.3389/fmicb.2022.858263

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Copyright © 2022 Kusada, Arita, Tohno and Tamaki.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reverse transcription-polymerase chain reaction analyses of lpBSH gene in L. paragasseri JCM 5343^T. The synthesized cDNA samples from total RNA of non-supplemented (lane 1), TDCA-supplemented (lane 2), and cephalosporin C-supplemented (lane 3) strains of JCM 5343^T cells were used as the template for PCR analysis, respectively. Sterilized water (lane N) and genomic DNA of strain JCM 5343^T (lane P) were used as negative and positive control templates, respectively. The 16S rRNA gene was used as internal standard control. Lane M, molecular size markers (100 bp DNA ladder, Promega, Madison, WI, United States).