Figure 2.

LN vessels and sinuses are delimited by endothelial cells expressing different levels of CD31, veCadherin and Lyve-1. Consecutive 10 µm thick sections from the same LN as in Figure 1 were stained with (A) anti-CD169, anti-veCadherin, anti-CD31 antibodies and DAPI or (B) anti-CD21, anti-Lyve-1, anti-CD31 antibodies and DAPI. Tb, Trabeculae; Eff Area, efferent area. F: B cell Follicle. Red arrows: T cell area veCadherin^pos vessels, pink arrows: Trabecular CD31^pos endothelial cells, yellow arrows: Trabecular Lyve-1^pos endothelial cells, double pink/yellow arrows: efferent area sinus endothelial cells, blue arrows: efferent area veCadherin^pos vessels, violet arrows: efferent sinus Lyve-1^pos/veCadherin^pos endothelial cells. The right pictures numbered 1 and 2 in A) and 3 and 4 in B) referred to enlargements of the framed regions 1 to 4 on the left large pictures. Whole LN pictures are individual images from 10x objective acquisitions, scale bar 500 µm. Images are representative of 3 LN from different animals.