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. 2022 Jun 6;13:869384. doi: 10.3389/fimmu.2022.869384

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Copyright © 2022 Dubreil, Ledevin, Hervet, Menard, Philippe, Michel, Larcher, Meurens and Bertho

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Trabecular lymphatic sinus and B cell follicles relative positioning 10 µm thick sections of a tracheobronchial LN were stained using (A) anti-CD169, anti-Lyve-1, anti-CD31 antibodies and DAPI; (B) anti-CD11c, anti-Lyve-1, anti-MHC-II and DAPI; (C) anti-Pax5, anti-Lyve-1, anti-Bcl6 and DAPI, (D) anti-CD169, anti-Ki67, anti-CD21 and DAPI; (E) anti- CD21, anti-Ki67, anti-Pax5 and DAPI; (F) anti- Pax5, anti-Ki67, anti- Bcl6 and DAPI. F, follicle; Tb, Trabeculae; pfMθ, perifollicular macrophages. Light blue arrow: CD169^pos macrophages, light green arrows: CD11c^pos/MHC-II^high dendritic cells, light yellow arrows: centrocytes. (A, B), scale bar 50µm, (C–F) scale bar 100µm. Whole LN pictures are individual images from 10x objective acquisitions. Images are representative of 3 LN from different animals.