TABLE 5.

Ruminal microbiota community difference between the two groups by using the Mann–Whitney U test.

Items	Groups		SEM	P-value
	RA	RAS
Phylum
Bacteroidota	38.38 ± 4.96	53.61 ± 1.41	2.963	0.001
Firmicutes	43.98 ± 2.77	30.70 ± 2.41	2.567	< 0.001
Euryarchaeota	3.16 ± 1.16	2.20 ± 0.92	0.387	0.240
Proteobacteria	2.68 ± 0.38	3.87 ± 0.59	0.263	0.011
Verrucomicrobiota	0.38 ± 0.13	0.43 ± 0.15	0.046	0.577
Chloroflexi	0.13 ± 0.04	0.32 ± 0.07	0.040	0.004
Synergistota	0.27 ± 0.05	0.90 ± 0.27	0.140	0.005
Desulfobacterota	0.68 ± 0.11	0.34 ± 0.08	0.069	0.002
Actinobacteriota	1.06 ± 0.28	0.28 ± 0.02	0.169	0.002
Spirochaetota	0.46 ± 0.19	1.00 ± 0.22	0.130	0.017
Genus
Prevotella	11.04 ± 0.10	8.10 ± 1.43	0.707	0.018
Methanobrevibacter	2.67 ± 0.74	1.20 ± 0.85	0.290	0.274
Quinella	0.56 ± 0.08	0.56 ± 0.35	0.080	0.993
Rikenellaceae_RC9_gut_group	6.73 ± 1.74	12.33 ± 0.79	1.240	0.004
Prevotellaceae_UCG-001	1.56 ± 0.31	1.39 ± 0.54	0.164	0.659
Succiniclasticum	3.66 ± 0.92	4.57 ± 0.50	0.337	0.203
NK4A214_group	2.72 ± 0.71	1.43 ± 0.25	0.311	0.018
Christensenellaceae_R-7_group	6.60 ± 1.11	4.07 ± 1.40	0.565	0.013
UCG-005	1.37 ± 0.46	1.08 ± 0.11	0.139	0.351
Ruminococcus	1.59 ± 0.41	3.93 ± 0.23	0.660	0.041