TABLE 6.
Differential metabolites in the rumen of yak calves in the RA and RAS groups.
| Metabolites | mzmed | rtmed | log2FC | P-value | VIP | Regulated |
| Saccharin | 181.992 | 6.042 | 7.160 | 0.000 | 3.387 | Up |
| 6-Keto-prostaglandin f1alpha | 369.228 | 12.182 | 2.556 | 0.009 | 2.381 | Up |
| delta-Tocopherol | 401.343 | 16.697 | 4.883 | 0.012 | 2.348 | Up |
| Pyridoxine O-Glucoside | 330.120 | 2.068 | 4.228 | 0.014 | 2.896 | Up |
| 2′-O-Methyluridine | 257.079 | 5.528 | 1.411 | 0.015 | 2.638 | Up |
| 15(S)-HpETE | 353.234 | 12.929 | 1.405 | 0.016 | 2.137 | Up |
| FAHFA (16:1/18:3) | 529.424 | 14.506 | 0.964 | 0.017 | 1.374 | Up |
| Isopentenyladenine | 202.110 | 10.799 | 2.845 | 0.025 | 2.731 | Up |
| N1-isopropyl-2-(1H-2-pyrrolylcarbonyl)-1-hydrazinecarboxamide | 419.212 | 9.289 | 2.629 | 0.031 | 1.717 | Up |
| N7-Methylguanosine | 298.115 | 1.361 | 0.638 | 0.033 | 1.566 | Up |
| Pepstatin | 684.455 | 15.539 | 0.884 | 0.033 | 2.031 | Up |
| (+/-)8(9)-DiHET | 337.239 | 13.317 | 1.122 | 0.038 | 2.039 | Up |
| Xylitol | 151.061 | 1.426 | 0.862 | 0.041 | 1.692 | Up |
| (+/-)9,10-dihydroxy-12Z-octadecenoic acid | 313.239 | 12.924 | 0.962 | 0.041 | 2.172 | Up |
| Cinnamoylglycine | 204.067 | 8.755 | 0.639 | 0.046 | 1.025 | Up |
| 3,5-Dihydroxybenzoic acid | 153.020 | 7.550 | –4.292 | 0.001 | 2.951 | Down |
| (3R)-8-hydroxy-3-(4-methoxyphenyl)-3,4-dihydro-1H-2-benzopyran-1-one | 269.083 | 9.621 | –3.107 | 0.002 | 3.140 | Down |
| 2-Hydroxyhippuric acid | 194.046 | 7.134 | –2.277 | 0.004 | 2.801 | Down |
| FAHFA (2:0/23:0) | 411.349 | 16.143 | –3.557 | 0.005 | 2.496 | Down |
| Hydroquinone | 109.030 | 7.551 | –3.315 | 0.006 | 2.674 | Down |
| D-Glucosyl-beta-1,1-N-palmitoyl-D-erythro-sphingosine | 698.560 | 16.209 | –1.790 | 0.007 | 2.303 | Down |
| 3-(methylsulfanyl)-5H-[1,2,4]triazino[5,6-b]indole | 215.039 | 10.572 | –1.497 | 0.010 | 2.102 | Down |
| PC (15:1/18:2) | 800.546 | 16.225 | –1.955 | 0.011 | 1.749 | Down |
| Isorhapontigenin | 257.082 | 10.283 | –3.028 | 0.016 | 2.153 | Down |
| FAHFA (2:0/18:1) | 357.265 | 13.102 | –0.751 | 0.017 | 1.655 | Down |
| 1-(2,4-dihydroxyphenyl)-2-(3,5-dimethoxyphenyl)propan-1-one | 301.108 | 7.583 | –2.793 | 0.018 | 1.856 | Down |
| 2-Furoylglycine | 168.031 | 5.992 | –2.379 | 0.019 | 2.147 | Down |
| PE (16:0/16:0) | 690.509 | 14.288 | –1.134 | 0.019 | 1.724 | Down |
| D-Glucuronic acid | 193.036 | 12.638 | –1.792 | 0.024 | 2.331 | Down |
| PG (20:0/20:4) | 825.567 | 15.518 | –3.036 | 0.032 | 2.263 | Down |
| PG (20:0/20:3) | 827.584 | 16.042 | –2.299 | 0.032 | 1.490 | Down |
| 13,14-dihydro Prostaglandin F1α | 393.243 | 14.550 | –1.955 | 0.033 | 2.025 | Down |
| PG (18:1/22:4) | 823.552 | 15.392 | –1.183 | 0.033 | 1.623 | Down |
| 3-methyl-4-[2-(2-methylphenyl)hydrazono]-4,5-dihydro-1H-pyrazol-5-one | 215.093 | 1.915 | –0.860 | 0.038 | 1.362 | Down |
| UMP | 323.029 | 1.460 | –1.210 | 0.039 | 2.018 | Down |
| N1-[4-(cyanomethyl)phenyl]-4-chlorobenzamide | 269.046 | 10.375 | –2.514 | 0.046 | 1.759 | Down |
| Catechin | 289.072 | 7.111 | –1.567 | 0.049 | 2.133 | Down |
FC, fold change; mzmed, mass-to-charge ratio of metabolites; rtmed, retention time of metabolites; VIP, variable importance in the projection.
Regulated “up” represents a higher abundance in calves in the RAS group, “down” represent a higher abundance in calves in the RA group.