Figure 4.

Delayed vascular development in GPR81-null mice brain is associated with a decreased VEGF expression and increased levels of the antiangiogenic TSP-1 factor. Real-time quantitative PCR and Western blot analysis of (a) TSP-1 (170 kDa) and (b) VEGF-A (42 kDa) expression levels in brain samples from WT and GPR81-null mice at different postnatal (P) ages. 18S and Vinculin (110 kDa) were used as internal controls for qPCR and Western blot respectively. Data represent mean ± SD; n = 5 animals per group; **p < 0.01, ***p < 0.001 compared to WT P5; one-way ANOVA followed by the Dunnett’s test for multiple comparison with control.