Table 1.
Primers used in this study.
| Gene | Primer direction a and sequence (5’-3’) | Product (bp b ) |
|---|---|---|
| Genotyping | ||
| cyp17a2 | F: GCAGCTGTCGTCTCAGTACG | 149 |
| R: GGAGTGTGCTCACCATCTTG | ||
| qPCR | ||
| pomca (9) | F: TCTTGGCTCTGGCTGTTC | 184 |
| R: TCGGAGGGAGGCTGTAG | ||
| lhβ (9) | F: AGCTTGGTTTTTCCACGCTG | 170 |
| R: TACGTGCACACTGTCTGGTG | ||
| fshβ (9) | F: AGAGCGAAGAATGTGGGAGC | 178 |
| R: GAATCAACCCCTGCAGGACA | ||
| gh1 (9) | F: GCATCAGCGTGCTCATCAAG R: TGAGACTGGTCTCCCCTACG |
114 |
| prl (9) | F: CTCAGCACCTCACTCACCAAT R: CAGAGACCGAGCCAATGACA |
168 |
| tshβ (9) | F: AGGTTGCCGTGCCTATGTG R: GGACCCACCAACTCCTTTATGT |
145 |
| cyp17a1 (9) | F: CTCTTTGACCCAGGACGCTT R: TTTGCAAAATCCACGCCAGG |
154 |
| β-actin (9) | F: ACTCAGGATGCGGAAACTGG | 119 |
| R: AGGGCAAAGTGGTAAACGCT | ||
| ef1α (16) | F: AAGATCGGCTACAACCCTGC | 107 |
| R: TTCCATCCCTTGAACCAGCC | ||
| gapdh (17) | F: GATACACGGAGCACCAGGTT | 158 |
| R: CAGGTCACATACACGGTTGC | ||
a
F, Forward; R, Reverse.
b
base pairs.