Figure 3.

HDAC inhibitor treatment increased the editing efficiency of both BE3 and ABE7.10

(A) Effect of nexturastat A, abexinostat, and vorinostat on C-to-T editing of BE3 at four loci. The control group was treated with DMSO. (B) Effect of nexturastat A and abexinostat on A-to-G editing of ABE7.10 at 7 loci. (C) Relative efficiency of the editing of BE3 or ABE7.10 by nexturastat A and abexinostat treatment to that by DMSO treatment. The efficiencies of both cytosine and adenosine base editing were quantified using Sanger sequencing and were analyzed using EditR. Each experiment was repeated at least three times. Data are represented as mean ± SD; asterisks indicate statistically significant differences between DMSO-treated cells and HDAC inhibitor-treated cells (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001). Base editing efficiencies were analyzed using Sanger sequencing and EditR calculation.⁴¹