Figure 4.

HDAC inhibitor treatment generally increased a wide range of CBEs

(A) The effect of nexturastat A and abexinostat treatment on the editing of the SaCas9-derived base editor BE3-SaKKH. The control group was treated with DMSO. (B) The effect of nexturastat A and abexinostat treatment on different cytosine deaminase-derived base editors, including AID-BE3, target-AID, and A3A-BE3. The control group was treated with DMSO. The editing efficiency was quantified using Sanger sequencing and was analyzed using EditR. Each experiment was repeated at least three times. Data are represented as mean ± SD; asterisks indicate statistically significant differences between DMSO-treated cells and HDAC inhibitor-treated cells (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001). Base editing efficiencies were analyzed using Sanger sequencing and EditR calculation.