Figure 5.

HDAC inhibitor treatment increased product purity of BE3

(A) Representative Sanger sequencing results and EditR analysis showing the effect of HDAC inhibitors on the editing of site 28 by BE3. (B) The effects of HDAC inhibitor treatment on the C-to-T (left panel) and C-to-G/A (right panel) conversions of BE3 at 4 genomic loci. (C) Quantification of the product distribution of (B). Base editing efficiency was quantified using Sanger sequencing and analyzed using EditR. Each experiment was repeated at least three times. Data are represented as mean ± SD; asterisks indicate statistically significant differences between DMSO-treated cells and HDAC inhibitor-treated cells (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001). Base editing efficiencies were analyzed using Sanger sequencing and EditR calculation.