Figure 1.

Overexpression α-Syn inhibits BDNF expression

(A) Western blot assay for p-CREB, CREB, BDNF, and MeCP2 in SH-SY5Y cells 24 h after GFP-α-Syn transfection (mean ± SEM, n = 4 per group, Student’s t test, ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001). (B) Protein expression of p-CREB, CREB, BDNF, and MeCP2 in the SNc of human AAV-α-Syn-treated mice (mean ± SEM, n = 6 per group, Student’s t test, ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001). (C) Protein expression of p-CREB, CREB, BDNF, and MeCP2 in the striatum from DLB patients and controls (mean ± SEM, n = 10 per group, Student’s t test, ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001). There was a positive correlation between BDNF levels and the ratio of phosphorylated CREB/CREB in DLB patients (n = 10). Furthermore, there was a negative correlation between BDNF levels and MeCP2 levels in DLB patients (n = 10).